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Figure 4. Influence of <t>COL7A1</t> on proliferation and migration of 786-O ccRCC cells. (a): RT-qPCR showing the expression level of COL7A1 in three RCC cell lines: 786-O, ACHN, and Caki-1 (n = 6). (b): Western blot of COL7A1 protein level in the three cell lines and the respective quantification relative to GAPDH (b′) (n = 3). (c,c′): Western blot of shCOL7A1 786-O cell lines generated with different shRNAs and its quantification (n = 3). (d): Proliferation assay showing doubling time of 786-O shCTRL, shRNA1, and shRNA5 cell lines (n = 4). €: Wound healing assay of 786-O shCTRL, shRNA1, and shRNA5 cell lines, graph represents wound confluence (%) 10 h after the scratch (n = 24). Statistical analysis was made using the unpaired t-test, p < * 0.05, ** 0.01, *** 0.001, **** 0.0001, ns: not significant.
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Figure 4. Influence of <t>COL7A1</t> on proliferation and migration of 786-O ccRCC cells. (a): RT-qPCR showing the expression level of COL7A1 in three RCC cell lines: 786-O, ACHN, and Caki-1 (n = 6). (b): Western blot of COL7A1 protein level in the three cell lines and the respective quantification relative to GAPDH (b′) (n = 3). (c,c′): Western blot of shCOL7A1 786-O cell lines generated with different shRNAs and its quantification (n = 3). (d): Proliferation assay showing doubling time of 786-O shCTRL, shRNA1, and shRNA5 cell lines (n = 4). €: Wound healing assay of 786-O shCTRL, shRNA1, and shRNA5 cell lines, graph represents wound confluence (%) 10 h after the scratch (n = 24). Statistical analysis was made using the unpaired t-test, p < * 0.05, ** 0.01, *** 0.001, **** 0.0001, ns: not significant.
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Figure 4. Influence of <t>COL7A1</t> on proliferation and migration of 786-O ccRCC cells. (a): RT-qPCR showing the expression level of COL7A1 in three RCC cell lines: 786-O, ACHN, and Caki-1 (n = 6). (b): Western blot of COL7A1 protein level in the three cell lines and the respective quantification relative to GAPDH (b′) (n = 3). (c,c′): Western blot of shCOL7A1 786-O cell lines generated with different shRNAs and its quantification (n = 3). (d): Proliferation assay showing doubling time of 786-O shCTRL, shRNA1, and shRNA5 cell lines (n = 4). €: Wound healing assay of 786-O shCTRL, shRNA1, and shRNA5 cell lines, graph represents wound confluence (%) 10 h after the scratch (n = 24). Statistical analysis was made using the unpaired t-test, p < * 0.05, ** 0.01, *** 0.001, **** 0.0001, ns: not significant.
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Image Search Results


Journal: Cell Genomics

Article Title: Multi-omics integration identifies cell-state-specific repression by PBRM1-PIAS1 cooperation

doi: 10.1016/j.xgen.2023.100471

Figure Lengend Snippet:

Article Snippet: Primary antibodies used include anti-PBRM1 (Bethyl, A700-019), anti-PIAS1 (D33A7) (Cell Signaling, 3550), and anti-COL7A1 (4D2) (Santa Cruz Biotechnology, sc-33710).

Techniques: Recombinant, SYBR Green Assay, Protease Inhibitor, In Situ, Blocking Assay, Isolation, Sequencing, shRNA, Software

Figure 4. Influence of COL7A1 on proliferation and migration of 786-O ccRCC cells. (a): RT-qPCR showing the expression level of COL7A1 in three RCC cell lines: 786-O, ACHN, and Caki-1 (n = 6). (b): Western blot of COL7A1 protein level in the three cell lines and the respective quantification relative to GAPDH (b′) (n = 3). (c,c′): Western blot of shCOL7A1 786-O cell lines generated with different shRNAs and its quantification (n = 3). (d): Proliferation assay showing doubling time of 786-O shCTRL, shRNA1, and shRNA5 cell lines (n = 4). €: Wound healing assay of 786-O shCTRL, shRNA1, and shRNA5 cell lines, graph represents wound confluence (%) 10 h after the scratch (n = 24). Statistical analysis was made using the unpaired t-test, p < * 0.05, ** 0.01, *** 0.001, **** 0.0001, ns: not significant.

Journal: Cancers

Article Title: COL7A1 Expression Improves Prognosis Prediction for Patients with Clear Cell Renal Cell Carcinoma Atop of Stage.

doi: 10.3390/cancers15102701

Figure Lengend Snippet: Figure 4. Influence of COL7A1 on proliferation and migration of 786-O ccRCC cells. (a): RT-qPCR showing the expression level of COL7A1 in three RCC cell lines: 786-O, ACHN, and Caki-1 (n = 6). (b): Western blot of COL7A1 protein level in the three cell lines and the respective quantification relative to GAPDH (b′) (n = 3). (c,c′): Western blot of shCOL7A1 786-O cell lines generated with different shRNAs and its quantification (n = 3). (d): Proliferation assay showing doubling time of 786-O shCTRL, shRNA1, and shRNA5 cell lines (n = 4). €: Wound healing assay of 786-O shCTRL, shRNA1, and shRNA5 cell lines, graph represents wound confluence (%) 10 h after the scratch (n = 24). Statistical analysis was made using the unpaired t-test, p < * 0.05, ** 0.01, *** 0.001, **** 0.0001, ns: not significant.

Article Snippet: Antibodies used: COL7A1 (Santa Cruz Technologies #sc33710; 1/1000e, Goa, India) and GAPDH (Life technologies #AM4300; 1/40,000e, Carlsbad, CA, USA)

Techniques: Migration, Quantitative RT-PCR, Expressing, Western Blot, Generated, Proliferation Assay, Wound Healing Assay